ABSTRACT
Objective To investigate the endogenous carnosine,glutathione system and oxidative stress level in serum of patients with diabetic cardiomyopathy(DCM),and the potential relationships among them.Meth-ods The serum of 102 healthy people,96 patients with type 2 diabetes mellitus and 74 patients with diabetic car-diomyopathy in the third affiliated hospital of QiQihar Medical University were enrolled.Carnosine content was mea-sured by ELISA.T-GSH,GSH and GSSG were detected by using micro-enzyme labeling assay.GSH-Px,GST and GRAC were detected by using colorimetry. The content of NO was detected by nitrate reduction method. The con-tent of H2O2was detected by using molybdic acid coloring method. The activities of NOS and CAT was determined by colorimetry. Results Compared with the healthy control group,the average level of human serum carnosine, GSH content,GRAC,GSH/GSSG ratio,GST activity and T-SH content in the DM and DCM group were signifi-cantly reduced(P < 0.05,respectively). NO content,H2O2and iNOS activity were increased,but CAT activity was decreased in DCM group.Conclusion Decreases of levels of serum carnosine and glutathione(GSH)and the imbalance of redox state were observed in patients with diabetic cardiomyopathy,which may promote the occur-rence and development of the diabetic cardiomyopathy.
ABSTRACT
Objective To investigate the effect of combination of methotrexate and Chinese herbs in treating placenta increta.Methods 42 cases with stable vital signs were divided randomly into observe group and control group.On the basis of intravenous injection of methotrexate,the observe group were treated with Shenghua decoction while the control group were not.Results 5 cases of the observe group were given hysterectomy because of massive hemorrhage,10 cases were given uterine curettage,the successful rate of conservative treatment was 76.2%.12 cases of the control group were given hysterectomy because of massive hemorrhage,8 cases were given uterine curettage,the successful rate of conservative treatment was 42.9%.After statistics comparison,there was significant difference(P＜0.05)between observe group and control group.Conclusion Combination of methotrexate and Chinese herbs iS a better therapy for placenta increta.